In 1874, Fanny Angelina married physician and bacteriologist Walther Hesse, an investigator of air quality and, specifically, air-borne microbes. In the Winter of 1880-81, Hesse became a research student with Koch in Berlin and experienced firsthand the difficulty of growing microbes on gelatine and the other growth media used at the time.
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Codex 5.3 after optimizing a principal component analysis implementation.